Nih Ravi is the well established breed of buffalo in Punjab. There are about 140 million buffaloes in the World out of which the largest buffalo population is in India, China and Pakistan. It is a multipurpose animal and fulfils milk and meat demand of mankind. In Pakistan it contributes about 74% of total milk yield. The annual rate of increase in buffalo population is 2.5% and increase in milk production is 3.5% in India and Pakistan. To fulfil the increasing demand of milk and meat, there is an immense need to improve the health status and production potentials of dairy animals. This can he achieved only if we are abreast with physiological norms, the disturbance of which lowers productivity. There are numerous problems of health, reproduction and milk production of Nih Ravi buffalo. Despite this very little information is available on blood chemistry of buffalo belonging to different agro-clirnatic conditions. There are various factors which play important role in their rate of production and minerals are one of those factors. Imbalances of minerals, produces deterimental effect on the performance of livestock e.g. Mineral imbalances lead to syndromes manifested in form of retarded growth, low production, late maturity, wasting disease, non infectious abortion, infertility, silent heat, retained placenta, dystokia and prolapse of vagina and uterus. To cope with the situation more research is required on the subject to locate the extent of mineral imbalances. The conclusive results will produce direct or indirect effect on the production and reproductive performance of livestock. The study is therefore planned to find out the serum mineral profile of lactating and non lactating buffaloes from different areas of Punjab and the effect of stage and number of lactation on mineral profile and to find the correlation of serum mineral profile with prolapse of vagina and uterus.
To determine the mineral profile in lactating and non lactating Nih Ravi buffaloes 200 blood samples were collected from Okara, Jhelum and Lahore/Sheikhupura. The serum was separated from blood by centrifugation. Serum was analyzed for sodium, potassium, chloride, calcium, magnesium, phosphorus and iron on spectrophotometer. The data thus obtained was subjected to statistical analysis by using analysis of variance and the differences in means were tested by using (L.S.D. test) least significant diference test.
The mean values of serum mineral in lactating and non lactating buffaloes on overall basis were subjected to statistical analysis and found non significant differences. However serum magnesium levels were found to be significant in lactating and non lactating buffaloes.
The means values of serum minerals in buffaloes of various districts were subjected to statistical analysis by using analysis of variance and L.S.D. test and significant differences were found between districts (P <0.05) for sodium, potassium, magnesium, phosphorus and iron, while non significant differences were found for calcium and chloride (P>0.05).
The mean values of serum mineral in lactating and non lactating buffaloes on the basis of lactation number were subjected to analysis of variance and L.S.D. test and found non significant differences for serum sodium, potassium, chloride, calcium, magnesium and iron, while serum phosphorus showed singificant differences on the basis of lactation numbers and stage of lactations.
The differences in the mean values of serum sodium, potassium, chloride, magnesium and phosphorus were found to be significant. While calcium and iron showed non significant differences within distircts.

The serum sodium showed deficiency in Lahore/Sheikhupura district while serum potassium, chloride, calcium, magnesium and iron values were found in normal range in all districts. However serum phosphorus level were slightly higher in buffaloes of all the districts.